منابع مشابه
A practical synthesis of the 13C/15N-labelled tripeptide N-formyl-Met-Leu-Phe, useful as a reference in solid-state NMR spectroscopy
A mild synthetic method for N-formyl-Met-Leu-Phe-OH (1) is described. After Fmoc solid phase peptide synthesis, on-bead formylation and HPLC purification, more than 30 mg of the fully (13)C/(15)N-labelled tripeptide 1 could be isolated in a typical batch. This peptide can be easily crystallised and is therefore well suited as a standard sample for setting up solid-state NMR experiments.
متن کاملGlutarate and N-acetyl-L-glutamate buffers for cell-free synthesis of selectively 15N-labelled proteins.
Cell-free protein synthesis provides rapid and economical access to selectively 15N-labelled proteins, greatly facilitating the assignment of 15N-HSQC spectra. While the best yields are usually obtained with buffers containing high concentrations of potassium L-glutamate, preparation of selectively 15N-Glu labelled samples requires non-standard conditions. Among many compounds tested to replace...
متن کاملComparison of the in situ degradation parameters of fresh and frozen-thawed 15N-labelled alfalfa and ryegrass
Degradation of dry matter (DM) and nitrogen (N) in fresh or frozen-thawed alfalfa and ryegrass was studied by using in situ technique. The forages were labeled with 15N during growth in a glasshouse, harvested at similar growth phase, and fresh (F) or frozen-thawed (FT) samples were incubated in the rumen of 3 sheep. There was no difference (P>0.05) between forage type (S) for the immediately s...
متن کاملOverhauser DNP with 15N labelled Frémy's salt at 0.35 Tesla.
The effectiveness of dynamic nuclear polarization (DNP) as a tool to enhance the sensitivity of liquid state NMR critically depends on the choice of the optimal polarizer molecule. In this study the performance of (15)N labelled Frémy's salt as a polarizing agent in Overhauser DNP is investigated in detail at X-band (0.35 T, 9.7 GHz EPR, 15 MHz (1)H NMR) and compared to that of TEMPONE-D,(15)N ...
متن کاملEfficient synthesis of norbuprenorphines coupled with enkephalins and investigation of their permeability
An efficient approach for the synthesis of norbuprenorphin derivatives through coupling of enkephalins and norbuprenorphine intermediate is described. Norbuprenorphine derivative was synthesized from thebaine and then, its reaction with succinic acid and phthalic acid was also studied. Meanwhile, the synthesis of enkephalins was done using solid phase peptide synthesis approach. Furthermore, af...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1995
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.49-0149